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ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; red=vaccines formulated with alum and CpG; orange=vaccines formulated with SWE. ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat) (ZRS04, ZRS05 see ). Alum=aluminium hydroxide; CpG=CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A; SWE=Squalene-in-Water-Emulsion.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; red=vaccines formulated with alum and CpG; orange=vaccines formulated with SWE. ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat) (ZRS04, ZRS05 see ). Alum=aluminium hydroxide; CpG=CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A; SWE=Squalene-in-Water-Emulsion.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Variant Assay, Emulsion

ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; red=vaccines formulated with alum and CpG; ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat) (see ). Antigens tested see . Alum=aluminium hydroxide; CpG=CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; red=vaccines formulated with alum and CpG; ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat) (see ). Antigens tested see . Alum=aluminium hydroxide; CpG=CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Variant Assay

ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; red=vaccines formulated with alum and CpG; ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat) (see ). Alum=aluminium hydroxide; CpG=CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; red=vaccines formulated with alum and CpG; ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat) (see ). Alum=aluminium hydroxide; CpG=CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Variant Assay

ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; MP; red=vaccines formulated with alum and CpG; ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat). (see ). Alum=aluminium hydroxide; CpG = CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS=Zoogenic Recombinant SARS; blue=vaccines formulated with alum and MPLA; MP; red=vaccines formulated with alum and CpG; ZRS03=Zoogenic Recombinant SARS version 3 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS2-variant [either alpha, beta or gamma] + SARS1-bat). (see ). Alum=aluminium hydroxide; CpG = CpG oligodeoxynucleotides; MPLA=Monophosphoryl-Lipid A.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Variant Assay

ZRS=Zoogenic Recombinant SARS; orange=vaccines formulated with SWE; ZRS04=Zoogenic Recombinant SARS version 4 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) (see ). Antigens tested see . SWE=Squalene-in-Water-Emulsion.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS=Zoogenic Recombinant SARS; orange=vaccines formulated with SWE; ZRS04=Zoogenic Recombinant SARS version 4 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) (see ). Antigens tested see . SWE=Squalene-in-Water-Emulsion.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Emulsion

a. Neutralisation of ACE2 receptor SARS-CoV2 interaction; b. SARS-CoV2 in vitro viral neutralisation. ZRS = Zoogenic Recombinant SARS; orange = vaccines formulated with SWE; ZRS04 = Zoogenic Recombinant SARS version 4 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) (see ). SWE = Squalene-in-Water-Emulsion.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: a. Neutralisation of ACE2 receptor SARS-CoV2 interaction; b. SARS-CoV2 in vitro viral neutralisation. ZRS = Zoogenic Recombinant SARS; orange = vaccines formulated with SWE; ZRS04 = Zoogenic Recombinant SARS version 4 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) (see ). SWE = Squalene-in-Water-Emulsion.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: In Vitro, Recombinant, Vaccines, Emulsion

ZRS = Zoogenic Recombinant SARS; orange = vaccines formulated with SWE; ZRS04 = Zoogenic Recombinant SARS version 4 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) (see ). SWE = Squalene-in-Water-Emulsion.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS = Zoogenic Recombinant SARS; orange = vaccines formulated with SWE; ZRS04 = Zoogenic Recombinant SARS version 4 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) (see ). SWE = Squalene-in-Water-Emulsion.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Emulsion

ZRS=Zoogenic Recombinant SARS; orange=vaccines formulated with SWE; ZRS05=Zoogenic Recombinant SARS version 5 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) and MERS (see ). ISO=isotonic formulation; Antigens tested see . SWE=Squalene-in-Water-Emulsion.

Journal: bioRxiv

Article Title: Design and development of a SARS and MERS Combination Vaccine

doi: 10.1101/2025.10.27.683653

Figure Lengend Snippet: ZRS=Zoogenic Recombinant SARS; orange=vaccines formulated with SWE; ZRS05=Zoogenic Recombinant SARS version 5 containing as antigens the S1 sub-unit of (SARS2-Wuhan + SARS1-bat) and MERS (see ). ISO=isotonic formulation; Antigens tested see . SWE=Squalene-in-Water-Emulsion.

Article Snippet: A SARS2 spike primary mouse antibody (CE5, Native Antigen Company, MAB12443) was included as a positive control, and normal mouse serum (Invitrogen, 10410) used as a negative control.

Techniques: Recombinant, Vaccines, Formulation, Emulsion